Disease:

Amelanotic melanoma

Origin:

C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).

Species:

Homo sapiens

Tissue:

Skin

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Tumorigenic; Modal no. 45.

Patient:

Male, Caucasian, 53 yrs of age

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility: