C3 ELISA Kit (Mouse)
Referencia OKIA00089
embalaje : 1x96wellplate
Marca : Aviva Systems Biology
Contact local distributor :
Teléfono : +1 850 650 7790
C3 ELISA Kit (Mouse) (OKIA00089)
| Datasheets/Manuals | Printable datasheet for C3 ELISA Kit (Mouse) (OKIA00089) |
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| COA Datasheet | Printable COA datasheet for OKIA00089 |
| Predicted Species Reactivity | Monkey | ||||||||||||||||
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| Product Format | In Solution | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||
| ELISA Kit Duration | 50 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology C3 ELISA Kit (Mouse) (OKIA00089) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Complement C3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Complement C3 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Complement C3 captured in the well. | ||||||||||||||||
| ELISA Kit Range | 3.125 ng/ml - 200 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The Complement factor 3 (C3) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C3 in biological samples of mice. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-C3 antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-C3 ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-C3 . 7. C3 CALIBRATOR One vial containing a lyophilized C3 calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 1.379 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Mouse | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | C3 |
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| Gene Full Name | complement component 3 |
| Alias Symbols | ASP, Plp, HSE-MSF, AI255234 |
| NCBI Gene Id | 12266 |
| Protein Name | Complement C3 |
| Description of Target | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity.C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. |
| Uniprot ID | P01027 |
