Bovine Vitamin D-binding protein(GC) ELISA kit
Referencia CSB-EL009306BO-24T
embalaje : 24T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
group-specific component (vitamin D binding protein)
Associated names
GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
Abbreviation
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 μg/mL-10 μg/mL
Sensitivity
0.039 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 82-92 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 84-96 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 90-100 | |||||
Recovery
| The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 103 | 100-106 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Bovine Vitamin D-binding protein (GC) ELISA Kit was designed for the quantitative measurement of Bovine Vitamin D-binding protein (GC) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.
Customer Reviews and Q&A
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Target Background
Function
(From Uniprot)
(From Uniprot)
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Gene References into Functions
- Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production.
Subcellular Location
Secreted.
Protein Families
ALB/AFP/VDB family
Database Links
KEGG:
STRING:
UniGene: