Bovine Trypsin ELISA Kit
Referencia CSB-EL018811BO-96T
embalaje : 96T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of bovine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:100 | Average % | 89 | ||||
Range % | 82-96 | |||||
1:200 | Average % | 98 | ||||
Range % | 91-105 | |||||
1:400 | Average % | 87 | ||||
Range % | 82-94 | |||||
1:800 | Average % | 93 | ||||
Range % | 86-98 | |||||
The recovery of bovine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 89 | 85-95 | ||||
EDTA plasma (n=4) | 93 | 89-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ||||||
pg/ml | OD1 | OD2 | Average | |||
4000 | 0.180 | 0.174 | 0.177 | |||
2000 | 0.275 | 0.267 | 0.271 | |||
1000 | 0.429 | 0.420 | 0.424 | |||
500 | 0.613 | 0.629 | 0.621 | |||
250 | 0.985 | 1.021 | 1.003 | |||
125 | 1.581 | 1.653 | 1.617 | |||
62.5 | 2.093 | 2.184 | 2.139 | |||
0 | 2.652 | 2.725 | 2.688 | |||
and FAQs
This Bovine Trypsin ELISA Kit was designed for the quantitative measurement of Bovine Trypsin protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
Customer Reviews and Q&A
There are currently no reviews for this product.