Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit
Referencia CSB-E17548B-24T
embalaje : 24T
Marca : Cusabio
Contact local distributor :
Teléfono : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Thyroid Stimulating Hormone(TSH)
Associated names
TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation
TSH
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.9 uIU/mL-60 uIU/mL
Sensitivity
0.225 μIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
Recovery
| The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-104 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Bovine TSH ELISA Kit was designed for the quantitative measurement of Bovine TSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 uIU/mL-60 uIU/mL and the sensitivity is 0.225 μIU/mL.
Citations
- D Horikami,Scientific reports,2022
- Yongjin Cao,Chinese Chemical Letters,2019
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism.
Gene References into Functions
- Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene.
- identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links
KEGG:
STRING:
UniGene: