Biotin-tyramide is a biotinylation reagent specifically designed for tyramide signal amplification (TSA) methodologies in biological imaging and detection. TSA is an enzyme-mediated amplification procedure where horseradish peroxidase (HRP), conjugated to target-specific antibodies, catalyzes the deposition of biotin-labeled tyramide onto nearby protein residues in fixed cells or tissue sections. The reaction deposites biotin moieties precisely at detection sites. These biotin deposits can subsequently be identified via streptavidin-conjugated detection systems.The localization of deposition around HRP leads to good resolution. Suitable for either fluorescence or chromogenic detection. Common TSA applications include immunohistochemistry (IHC) and in situ hybridization (ISH).