BCKD Polyclonal Antibody
Referencia MBS8513727-0,1mL-AF647
embalaje : 0.1mL(AF647)
Marca : MyBiosource
Contact local distributor :
Teléfono : +1 850 650 7790
Western Blot analysis of 3T3 cells using BCKD Polyclonal Antibody.
Western Blot analysis of various cells using BCKD Polyclonal Antibody.
BCKD Antibody
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8513727MBS8513727 is an antibody targeting BCKD. Also known as BCKD Polyclonal Antibody. Reactive in Human, Mouse, RatPredicted Cross-Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus. More details
Product Overview
Online Datasheet in PDFProduct Name
BCKD, Antibody
Full Product Name
BCKD Polyclonal Antibody
Product Gene Name
anti-BCKD antibody
Product Synonym Names
[3 methyl 2 oxobutanoate dehydrogenase [Iipoamide]] kinase, mitochondrial; [3-methyl-2-oxobutanoate dehydrogenase [Iipoamide]] kinase; BCKD kinase; BCKD-kinase; BCKD_HUMAN; BCKDHKIN; Bckdk; BCKDKD; BDK; Branched chain alpha keto acid dehydrogenase kinase; Branched chain ketoacid dehydrogenase kinase; Branched-chain alpha-ketoacid dehydrogenase kinase; mitochondrial
Species Reactivity
Human, Mouse, Rat
Predicted Cross-Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus
Predicted Cross-Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus
Form/Format
Phosphate buffered saline , pH7.4, 150mM NaCI, 0.02% sodium azide and 505 glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin
Concentration
1 mg/ml (lot specific)
Specificity
BCKD Polyclonal Antibody detects endogenous levels of BCKD protein
Preparation and Storage
Store at -20°C . Stable for 12 months from date of receipt.
Applications Tested/Suitable for anti-BCKD antibody
Western Blot (WB), Immunohistochemistry paraffin (IHC-p), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-BCKD antibody
Western Blot: 1:1000-3000
Immunohistochemistry: 1/100 - 1/300
Immunocytochemistry (ICC): 1200-500
Immunofluorescence: 1:200-500
ELISA: 1:20000-1:40000
Not yet tested in other applications.
Immunohistochemistry: 1/100 - 1/300
Immunocytochemistry (ICC): 1200-500
Immunofluorescence: 1:200-500
ELISA: 1:20000-1:40000
Not yet tested in other applications.
Other Notes
Small volumes of anti-BCKD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for BCKD. It may not necessarily be applicable to this product.
NCBI Official Full Name
[3-methyl-2-oxobutanoate dehydrogenase
NCBI Official Synonym Full Names
branched chain ketoacid dehydrogenase kinase
NCBI Official Symbol
BCKDK
NCBI Official Synonym Symbols
BDK; BCKDKD
UniProt Protein Name
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
UniProt Synonym Protein Names
Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN
Protein Family
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase
UniProt Gene Name
BCKDK
UniProt Synonym Gene Names
BCKD-kinase; BCKDHKIN
NCBI Protein Information
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; 3-methyl-2-oxobutanoate dehydrogenase [lipoamide] kinase, mitochondrial
NCBI Summary for BCKD
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
Show more UniProt Comments for BCKD
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with anti-BCKD antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neurodegenerative Diseases Antibodies | >1 publications with BCKD and Neurodegenerative Diseases |
Organs/Tissues associated with anti-BCKD antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >3 publications with BCKD and Brain |
| Blood Antibodies | >2 publications with BCKD and Blood |
| Liver Antibodies | >2 publications with BCKD and Liver |
| Muscle Antibodies | >1 publications with BCKD and Muscle |
| Kidney Antibodies | >1 publications with BCKD and Kidney |