Images
Component A-labeled vesicles are induced by starvation in HeLa cells.
Note

Component A-labeled vesicles are induced by starvation in HeLa cells.

Component A-labeled vesicles are induced by starvation in HeLa cells.

Hela cells were incubated in a regular DMEM medium as control (A) or in a serum-depleted medium as autophagy treatment (B) for 16 hours. Both control cells and starved cells were incubated with component A for 30 minutes in a 37°C, 5% CO2 incubator, and then washed four times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel. Autophagy is indicated by bright blue dot staining of autophagic vacuoles (B).

  • Specifications

    Product Description

    Autophagy Assay Kit is used for the measurement of autophagy.

    Suitable Sample

    Culture cells

    Excitation/<br>Emission (nm)

    330/520 nm

    Detection Method

    Fluorometric

    Instrument Platform

    Fluorescence microscope, Fluorescence microplate reader

    Regulation Status

    For research use only (RUO)

    Storage Instruction

    Store the kit in desiccated environment at -20°C and avoid from light.

    Note

    Component A-labeled vesicles are induced by starvation in HeLa cells.

  • Applications

    Quantification

  • Publication Reference
eProcurement