Anti-TCR beta Antibody-APC labled
Referencia CFB8998-100
embalaje : 100Assays
Marca : Cohesion Biosciences
| Product Name: | Anti-TCR beta Antibody-APC labled |
| Cat No: | CFB8998 |
| Source: | Armenian Hamster |
| Reactivity: | M |
| Applications: | IF, FC |
| *Application Key: | |
| H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
| *Species Reactivity Key: | |
| E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference | |
Description:Armenian hamster monoclonal antibody APC labled to TCR betaImmunogen:Affinity purified TCR from mouse DO-11.10 cellsPurification:The antibody was purified by affinity chromatography.Specificity:Recognizes mouse TCR betaClonality:Monoclonal (clone: H57-597)Conjugation:APCForm:Armenian Hamster IgG. Liquid in PBS, pH 7.3, and 0.02% sodium azide.Dilution:10 μl / assayGene Symbol:TCRBAlternative Names:Tib; TCRbeta Entrez Gene (Mouse): 21577; Directions for Use:1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Storage/Stability:Shipped and store at 4°C for one year. Do not freeze.

