Anti-SNCA - Alpha-synuclein
Referencia AS08358
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Anti-SNCA | Alpha-synuclein
AS08 358 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
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| Immunogen: | recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 100 µl |
| Reconstitution: | For reconstitution add 100 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | ELISA (ELISA), Immunolocalization (IL), Western blot (WB) |
| Recommended dilution: | 1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB) |
| Expected | apparent MW: | 14 kDa |
| Confirmed reactivity: | Human |
| Predicted reactivity: | Primates, Chimpanzee, Gorilla |
| Western analysis
1. Cell lysate lacking alpha-synuclein 10 000 ng (negative control) |
| Additional information: | Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit. This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA. |
| Background: | Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. |
| Selected references: | Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327. Brännström et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014. |