Anti-Mouse IgD [Clone 11-26c.2a] - Biotin

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 28°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

FC,
IF,
IHC,
IP

Clone 1126c.2a specifically reacts with the delta heavy chain of mouse IgD, without cross reacting with other classes of mouse immunoglobulin like IgA, IgG, or IgM.

Immunoglobulin D (IgD) is one of the five main classes of immunoglobulins. Though it is an antibody that is not well understood, it has been discovered to have a vital role in both innate and adaptive immunity. It aids in activating and regulating B cells, especially in the upper respiratory mucosa. In this location, it enhances immune surveillance and defense against respiratory pathogens¹. IgD also helps maintain a healthy microbiome by equipping myeloid effector cells with IgD antibodies that react to mucosal antigens, including both commensal and pathogenic microbes².

The 1126c.2a clone is an essential tool in immunology research, particularly in the study of B cell development and activation pathways. It is widely used in flow cytometric analysis to identify surface IgD expression on mouse B cells. This provides valuable insights into B cell populations and their roles in immune responses^3,4. When the antibody is conjugated, it allows for easy detection and multiplexing in flow cytometry, enabling comprehensive immunophenotyping studies^5,6.

IgD is expressed by peripheral mature B cells.

1. Chen K, Xu W, Wilson M, et al. Nat Immunol. 2009;10(8):889898.
2. Gutzeit C, Chen K, Cerutti A. Eur J Immunol. 2018;48(7):11011113.
3. Böhmer RM, King NJ. Cytometry. 1984;5(5):543546.
4. Olejniczak SH, Stewart CC, Donohue K, Czuczman MS. Immunol Invest. 2006;35(1):93114.
5. Pearson T, Galfrè G, Ziegler A, Milstein C. Eur J Immunol. 1977;7(10):684690.
6. T B, G K, A K, A F, E U, P N. Journal of immunological methods. 1998;214(12).
7. Hamilton AM, Lehuen A, Kearney JF. Int Immunol. 1994;6(3):355361.
8. Ishihara K, Wood WJ, Wall R, et al. J Immunol. 1993;150(6):22532262.
9. Nitschke L, Kosco MH, Köhler G, Lamers MC. Proc Natl Acad Sci U S A. 1993;90(5):18871891.
10. Campbell KS, Cambier JC. EMBO J. 1990;9(2):441448.