Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Anti-METTL3 Antibody

Anti-METTL3 Antibody

Imprimir Imprimir
Por favor conéctese

Referencia CQA1783-30ul

embalaje : 30ul

Marca : Cohesion Biosciences

Contact local distributor :


Teléfono : +1 850 650 7790

  • Western blot analysis of METTL3 expression in HepG2 (A), mouse spleen (B) whole cell lysates. (Predicted band size: 25; 64 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of METTL3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of METTL3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product Name:Anti-METTL3 Antibody
Cat No:CQA1783
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to METTL3
Immunogen：Recombinant full length protein of human METTL3
Purification：The antibody was purified by immunogen affinity chromatography.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/20 - 1/50)
Gene Symbol：METTL3
Alternative Names：MTA70; N6-adenosine-methyltransferase 70 kDa subunit; MT-A70; Methyltransferase-like protein 3
Entrez Gene (Human)： 56339;
Entrez Gene (Mouse)： 56335;
SwissProt (Human)： Q86U44;
SwissProt (Mouse)： Q8C3P7;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Usted podría estar interesado también en los siguientes productos:



Referencia
Descripción
Cond.
Precio Sin IVA
NB-64-69258-10mg
SAH [979-92-0]
 10mg 
NB-64-45415-5mg
STM2457 [2499663-01-1]
 5mg 
NB-64-42870-1mg
STM2120 [2762286-04-2]
 1mg