Anti-LY6C Antibody Picoband®
Referencia A33313
embalaje : 100ug/vial
Marca : Boster Bio
Contact local distributor :
Teléfono : +1 850 650 7790
Tested Applications
Ly6c1/2 antibody was positively detected by WB in:
mouse RAW2647 whole cell, mouse ANA-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ly6c1/2 antibody was positively detected by IHC in:
rat spleen tissue, mouse spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Ly6c1/2 antibody was positively detected by FC in:
mouse spleen tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-LY6C Antibody Picoband®
View all LY6C1/2 Antibodies
View all LY6C1/2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse LY6C recombinant protein (Position: L27-G109).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated Molecular Weight
14.2 kDa
Background of Ly6c1/2
Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.
Cite This Product
Anti-LY6C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33313)
Protocols
Western Blot protocol for Ly6c1/2 antibody
Immunohistochemistry (IHC) protocol for Ly6c1/2 antibody
Flow Cytometry (FC) protocol for Ly6c1/2 antibody
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