Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) FITC Conjugated
Referencia M01106-4-FITC
embalaje : 100ug/vial
Marca : Boster Bio
Contact local distributor :
Teléfono : +1 850 650 7790
Tested Applications
ARG1 antibody was positively detected by WB in:
rat liver tissue, mouse liver tissue, monkey liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ARG1 antibody was positively detected by FC in:
Jurkat cell, SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Monkey, Mouse, Rat
Flow Cytometry (Fixed)
1-3μ/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12)
View all ARG1 Antibodies
View all ARG1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
2B12
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
35 kDa
Calculated Molecular Weight
34.7 kDa
Background of ARG1
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01106-4)
Protocols
Western Blot protocol for ARG1 antibody
Flow Cytometry (FC) protocol for ARG1 antibody
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