Anti-High Molecular Weight E. coli Protein Host Cell Protein (HCP) Antibody, 100 ug
Size:
100 ug 25 ul
Conjugation:
Unconjugated
2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 µg HMW HCP. Primary antibody: Rabbit anti-HMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.
Specifications
Product Data
Application
ELISA, WB
Recommended Dilution
WB: 1:500-1:1000 ELISA: 1:1000-1:2000
Reactivity
Escherichia coli
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).
Specificity
HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration
1.38 mg/ml - lot specific
Conjugation
Unconjugated
Storage
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stability
Expiration date is one (1) year from date of receipt.
Shipping
Blue Ice
Background
Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
