Anti-High Molecular Weight E. coli Protein Host Cell Protein (HCP) Antibody, 100 uL, Serum
Size:
100 ul
Conjugation:
Unconjugated
2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 µg HMW HCP. Primary antibody: Rabbit anti-HMW-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block: MB-070 for 1 hour at RT.
Specifications
Product Data
Application
ELISA, WB
Recommended Dilution
WB: 1:500 - 1:1000 ELISA: 1:1000 - 1:2000
Reactivity
Escherichia coli
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).
Specificity
This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide
Concentration
77.5 mg/mL - lot specific
Conjugation
Unconjugated
Storage
Store HMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability
Expiration date is one (1) year from date of receipt.
Shipping
Blue Ice
Background
Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been analyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
