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> Anti-DNMT1 Antibody

Anti-DNMT1 Antibody

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Referencia CQA6613-100ul

embalaje : 100ul

Marca : Cohesion Biosciences

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Teléfono : +1 850 650 7790

  • Western blot analysis of DNMT1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 144; 183; 184 kD; Observed band size: 200 kD)
  • Immunohistochemical analysis of DNMT1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of DNMT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-DNMT1 Antibody
Cat No:CQA6613
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to DNMT1
Immunogen：Recombinant protein of human DNMT1
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of DNMT1 protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/100)
Gene Symbol：DNMT1
Alternative Names：AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
Entrez Gene (Human)： 1786;
Entrez Gene (Mouse)： 13433;
SwissProt (Human)： P26358;
SwissProt (Mouse)： P13864;
SwissProt (Rat)： Q9Z330;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.