Anti-Claudin-5 Polyclonal Antibody 2
Referencia NB-64-113018-50uL
embalaje : 50uL
Marca : Neo Biotech
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Anti-Claudin-5 Polyclonal Antibody 2
(Synonyms: Transmembrane protein deleted in VCFS, TMDVCF, CLDN5, Claudin5, AWAL TMVCF) Copy Product InfoSynonyms: Transmembrane protein deleted in VCFS, TMDVCF, CLDN5, Claudin5, AWAL TMVCF
Catalog No. TMAB-00445 Copy Product Info
Anti-Claudin-5 Polyclonal Antibody 2 is a Rabbit antibody targeting Claudin-5. Anti-Claudin-5 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Claudin-5 Polyclonal Antibody 2 is a Rabbit antibody targeting Claudin-5. Anti-Claudin-5 Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Transmembrane protein deleted in VCFS, TMDVCF, CLDN5, Claudin5, AWAL TMVCF |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Claudin-5 Polyclonal Antibody, Unconjugated (TMAB-00445) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat penis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Claudin-5 Polyclonal Antibody, Unconjugated (TMAB-00445) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: human kidney cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Claudin Polyclonal Antibody, Unconjugated (TMAB-00445) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane 1: Recombinant human Claudin 5 protein, N-Trx-His Primary: Anti-Claudin 5 (TMAB-00445) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 30 kDa  , , , |
| Application | IF IHC-Fr IHC-P WB |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed in right lung and 192 other cell types or tissues |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008] |
| Immunogen | KLH conjugated synthetic peptide: human claudin 5 |
| Antigen Species | Human |
| Gene Name | CLDN5 |
| Gene ID | 7122 |
| Protein Name | Claudin-5 |
| Uniprot ID | O00501 |
| Biology Area | Tight Junctions |
| Function | Plays a major role in tight junction-specific obliteration of the intercellular space. |
| Molecular Weight | Theoretical: 24 kDa. Actual: 30 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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