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> Anti-cdc2, p34 (HRP) Monoclonal Antibody

Anti-cdc2, p34 (HRP) Monoclonal Antibody

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Referencia C2549-50P-HRP-100ul

embalaje : 100ul

Marca : US Biological

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C2549-50P-HRP cdc2, p34 (HRP)

Clone Type
Polyclonal
Host
mouse
Source
Xenopus
Isotype
IgG2a,k
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Ch Hu Rd
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Inhibition and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 5ug. Capable of immunoprecipitating cyclin protein associated with p34cdc2.
Inhibition: Inhibits the activation of p34cdc2 kinase by cyclin proteins.
Immunohistochemistry (Frozen): 5-10ug/ml.
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: A431 and HeLa 3T3 cell lysates; Xenopus egg extracts.
Immunohistochemistry: Tested on a variety of human tissues including tonsil (germinal center), spermatogonia and skin (basal layer).

Epitope Location:
The epitope core residues are thought to reside within amino acids 220-227 of the mouse p34cdc2 protein corresponding to the sequence LGTPNNEV.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 3G92|Host: mouse|Source: Xenopus|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2.|Specificity: Recognizes Xenopus p34cdc2. Does not appear to crossreact with the related p33cdk2 protein. Inhibits the activation of p34cdc2 kinase by cyclin proteins. Species Crossreactivity: Human, rodent, amphibian and chicken.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Xenopus p34cdc2. Does not appear to crossreact with the related p33cdk2 protein. Inhibits the activation of p34cdc2 kinase by cyclin proteins. Species Crossreactivity: Human, rodent, amphibian and chicken.
References
1. Kobayashi, H., et al., Mol. Biol. Cell. 3: 1279-1294 (1992). 2. Doussis-Anagnostopoulou, I.A., et al., Histopathology 24: 335-340 (1994).