Anti-ACTN3 Antibody Picoband®

Referencia PB10026

embalaje : 100ug/vial

Marca : Boster Bio

Contact local distributor :


Teléfono : +1 850 650 7790

Tested Applications

ACTN3 antibody was positively detected by WB in:

human HT1080 whole cell, rat skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, mouse skeletal muscle tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

ACTN3 antibody was positively detected by IHC in:

mouse skeletal muscle tissue, rat skeletal muscle tissue, human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

ACTN3 antibody was positively detected by IF in:

mouse skeletal muscle

ACTN3 antibody was positively detected by FC in:

WISH cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.

Product Information

Product Name
Anti-ACTN3 Antibody Picoband®
View all ACTN3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
103 kDa
Calculated Molecular Weight

103.2 kDa

Background of ACTN3
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.
Cite This Product
Anti-ACTN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10026)

Protocols

Western Blot protocol for ACTN3 antibody
Immunohistochemistry (IHC) protocol for ACTN3 antibody
Immunofluorescence (IF) protocol for ACTN3 antibody
Flow Cytometry (FC) protocol for ACTN3 antibody

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