Alpha-Amylase Inhibitor Screening Kit
Referencia E-BC-D018-48T
embalaje : 48T
Marca : Elabscience
Contact local distributor :
Teléfono : +1 850 650 7790
Diabetes research target
Alpha-Amylase Inhibitor Screening Kit (E-BC-D018)
For research use only.
| Detection Principle | α-Amylase in human body can not only act on the end of starch, but also act on the α-1,4 glucoside bond inside starch molecules. The degradation products are glucose, maltose and dextrin containing α-1,6 glucoside bond branch chain, which plays an important role in the digestion of polysaccharide compounds in food. Inhibition of α-amylase activity can not only reduce the digestion and absorption of starch, but also allow undigested and absorbed starch to enter the large intestine, promote gastrointestinal motility, and further reduce the occurrence of type 2 diabetes, which is an important drug target for the treatment of diabetes. The detection principle of this kit: α-Amylase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 405 nm. The addition of an inhibitor suppresses the activity of α-Amylase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference. |
| Sample Type | Inhibitor |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 7.2-10.0% | intra-assay CV: 0.4-1.3% |
| Sample Volume | 30 μL |
| Assay Time | 40 min |
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