Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Immunofluorescence
Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKT1 is approximately 3ng/ml as a capture antibody.
Immunoprecipitation
Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKT1 MaxPab rabbit polyclonal antibody.
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11 Western Blot analysis of AKT1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in HepG2 ( Cat # L019V1 ).
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in MCF-7 ( Cat # L046V1 ).
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in NIH/3T3(Cat # L018V1 ).
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in PC-12(Cat # L012V1 ).
Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in Raw 264.7(Cat # L024V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody (M03), clone 2E11.
Lane 1: AKT1 transfected lysate(55.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (78.54 KDa) .
- Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant AKT1.
Immunogen
AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]Immunofluorescence
Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20 ug/ml]ELISA
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKT1 is approximately 3ng/ml as a capture antibody.Immunoprecipitation
Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKT1 MaxPab rabbit polyclonal antibody.Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11 Western Blot analysis of AKT1 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in HepG2 ( Cat # L019V1 ).Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in PC-12(Cat # L012V1 ).Western Blot (Cell lysate)
AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in Raw 264.7(Cat # L024V1 ).Western Blot (Transfected lysate)
Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody (M03), clone 2E11.
Lane 1: AKT1 transfected lysate(55.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
- Gene Info — AKT1
Entrez GeneID
207GeneBank Accession#
BC000479Protein Accession#
AAH00479.1Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
- Interactomes
- Pathways
- Diseases