ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1].
Dilution : 1:1000

IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100