A673 cells
Referencia C0016074
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Disease:
Sarcoma
Origin:
This cell line was derived from metastatic site: ascites.
Species:
Mus musculus, mouse
Tissue:
Sarcoma
Morphology:
Suspension
Properties:
Suspension
Strain:
Swiss Webster
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37°C. Change medium every 2-3 days.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
References for Sarcoma 180 cells:
1. DUNHAM LJ, STEWART HL. A survey of transplantable and transmissible animal tumors. J Natl Cancer Inst. 1953 Apr;13(5):1299-1377. PMID: 13035452