3D FECT 1000 TRANSFECTION REAGENT

Referencia TF21000

embalaje : 1mL

Marca : OZ Biosciences

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Teléfono : +1 850 650 7790

  • 3D-Fect

3D-Fect

3D-Fect™ is suitable for all kinds of scaffolds and cells. This 3D Transfection Reagent is specifically developed to directly transfect cells cultured in 3D scaffolds. This reagent is based on a novel technology that adds a third dimension to cell culture and transfection. 3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behaviours.

Because 3D matrices are routinely used in basic research and therapeutic applications, OZ Biosciences has developed 3D-Fect™ Transfection Reagent specific for 3D-scaffolds. In this way, scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. This method allows studying tissue engineering and regeneration, tumour invasion, neural differentiation, cellular polarization, tissue formation,colonization, neurite growth...

 

3D-Fect is also designed for gene silencing applications, please refer to 3D-Fect protocol for siRNA transfection

 

  • Highly efficient: cell lines and primary cells
  • Specific for 3D Scaffolds
  • Compatible with all types of nucleic acids
  • Completely biodegradable
  • Long term protein expression
  • Serum Compatible

 

Sizes:

  • 250 µL: Good for 65 to 125 transfections with 1 µg of DNA
  • 500 µL: Good for 125 to 250 transfections with 1  µg of DNA
  • 1000 µL: Good for 250 to 500 transfections with 1 µg of DNA

  

Storage: +4°C

Shipping Conditions: Room temperature

 

Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Acinar Cells siRNA si3D-FectIN Parte S. Gastroenterology . 2024 May;166(5):842-858.e5. AdSC 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. ATDC5 siRNA si3D-FectIN Mori H. Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5. Chondrocytes siRNA si3D-FectIN Mori H. Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5. Chondrocytes 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. CLU-301 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. COS-1 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. COS-1 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. COS-7 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. COS-7 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Fibroblasts DNA 3D-Fect Toro Runzer C. Mol Ther Nuc Acid. 2023 May HEK-293 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. HeLa 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. HeLa 3D Transfection siRNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. HMEC-1 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. HMEC-1 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. MDCK 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. MEF 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Mesenchymal Stem Cells mRNA 3D-FectIN García Fuentes M. Thesis 2024 Mesenchymal Stem Cells DNA 3D-Fect Toro Runzer C. Mol Ther Nuc Acid. 2023 May Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM. Acta Biomater . 2020 May 15;S1742-7061(20)30224-5. Mesenchymal Stem Cells mRNA 3D-FectIN Ledo AM. Biomaterials. 2020 Jul;247:120016. Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Plank C. Eur. J. Nanomed. 2012;4(1):17–32 Mesenchymal Stem Cells 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Neural Stem Cells 3D Transfection DNA 3D-Fect Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Neural Stem Cells 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. NIH-3T3 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. Osteoblasts DNA 3D-Fect Toro Runzer C. Mol Ther Nuc Acid. 2023 May RAW 264.7 3D Transfection DNA 3D-FectIN Sapet C. Ther Deliv. 2013 Jun;4(6):673-85. RWPE1 siRNA 3D-Fect Laperrousaz B. Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030. RWPE1 siRNA 3D-FectIN Laperrousaz B. Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

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Protocol 3D-Fect

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MSDS 3D-Fect

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Results 3D-Fect

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3DFect Protocol for siRNA

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3D Transfection Brochure

Download 1.95MB

Results 3D-Fect gene silencing

Download 815.33KB

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