Manual| Component | AS221-01/11 | AS221-02/12 |
| 2×TransStart® FastPfu PCR SuperMix (-dye)/ (+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 1 ml | 5 ml |
2×TransStart FastPfu PCR SuperMix (+dye)
Referencia AS221-12
embalaje : 5×1ml
Marca : TransGen Biotech
Contact local distributor :
Teléfono : +1 850 650 7790
Product Details
TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O for amplification.The amplification products are blunt-ended. The amplification products of 2× TransStart® FastPfu PCR SuperMix (-dye) can be directly cloned into pEASY®-Blunt series vectors. The amplified product of 2×TransStart® FastPfu PCR SuperMix (+dye) can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning.
Applications
• High fidelity PCR
• Site-directed mutagenesis
• Blunt end cloning
• Complex templates
• GC/AT-rich templates
• Long fragment amplification
Storage
at --18℃ or below for two years
Shipping
Dry ice (-70 ℃)
Product Composition
References
1 Song R, Guo P, Ren X, et al. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer[J]. Molecular Cancer, 2023.(IF 37.30)
2 Jin S, Lin Q, Gao Q, et al. Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)[J]. Nature Protocols, 2022.(IF 17.02)
3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)
4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
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