Flow Cytometry
Flow cytometry of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells were treated with or without 100 uM Phloretin at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. To prepare adherent CHO-K1 cells for flow cytometry, EDTA was used to detach cells after staining. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
Example Data Analysis and Figures.
Example Data Analysis and Figures.
Fluorescence images of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells at 40,000 cells/well/100 uL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 20 mM Glucose (B) or 100 uM Phloretin (C) at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. Untreated control cells were stained under the same conditions. The fluorescence signal was measured using a fluorescence microscope with FITC filter.
- Specifications
Product Description
2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a 488 nm laser and 530/30 nm emission filter (FITC channel). This Assay Kit is the most robust tool for monitoring glucose transporters.
Suitable Sample
Cultured Cells.
Detection Method
Fluorimetric
Platform
Instrument: Flow cytometer
Excitation: 488 nm laser
Emission: 530/30 nm filter
Instrument specification(s): FITC channel
Instrument: Fluorescence microscope
Excitation: FITC filter
Emission: FITC filter
Recommended plate: Black wall/clear bottomRegulatory Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Example Data Analysis and Figures.
- Applications
Functional Study
Flow Cytometry
Flow cytometry of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells were treated with or without 100 uM Phloretin at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. To prepare adherent CHO-K1 cells for flow cytometry, EDTA was used to detach cells after staining. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel. - Publication Reference
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