α-Amanitin [23109-05-9]
Referencia A4548-1mg
embalaje : 1mg
Marca : APExBIO Technology
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Teléfono : +1 850 650 7790
α-Amanitin (CAS 23109-05-9) is a cyclic peptide toxin isolated from Amanita mushrooms, known primarily for its inhibition of eukaryotic RNA polymerase II. Mechanistically, α-Amanitin binds specifically and with high affinity to RNA polymerase II, preventing nucleic acid transcription elongation and consequently blocking mRNA synthesis. Due to this precise molecular inhibitory function, α-Amanitin serves as a widely-employed biochemical tool in vitro and in cell-based assays, facilitating the study of transcriptional regulation, RNA polymerase function, and gene expression pathways. Additionally, its selective inhibition properties are utilized experimentally to dissect complex biological processes dependent upon RNA polymerase II-mediated transcription in cellular research models.
- 1. Yuxi Gu, Yidan Song, et al. "The biogenesis of extrachromosomal circular DNA is associated with enhancer-promoter dynamics and chromatin architecture." Int J Biol Macromol. 2026 Apr 30:364:152307. PMID: 42067092
- 2. Tao Liu, Xu Zhang, et al. "GSTA1 Depletes Glutathione and Exacerbates Oxidative Stress in α-Amanitin-Induced Hepatotoxicity." Chem Biol Interact. 2026 Mar 29:433:112058. PMID: 41916443
- 3. Xiaofang Li, Lingling Guo, et al. "From Computationally Aided Hapten Design to Fluorescent Biosensing: A Novel Strategy for Highly Sensitive Simultaneous Detection of Amatoxins and Phallotoxins in Mushrooms." J Agric Food Chem. 2026 Apr 1;74(12):10550-10560. PMID: 41847769
- 4. Jing Wang, Wang Li, et al. "Natural degradation of RNA polymerase II is essential for oocyte chromatin reorganization and maternal-to-zygotic transition." Nat Commun. 2025 Dec 12;17(1):720. PMID: 41387441
- 5. Chunhui Zhu, Gang Liu, et al. "tRF16 affects NFKBIA stability and promotes osteoarthritis progression by regulating ALKBH5 expression in m6A-dependent manner." Commun Biol. 2025 Jun 5;8(1):868. PMID: 40473927
- 6. Jinfang Xue, Xiran Lou, et al. "Ezetimibe Protects Against Alpha - Amanitin - Induced Hepatotoxicity by Targeting the NTCP Receptor: Mechanistic Insights from In Vitro and In Vivo Models." Toxicon. 2025 Sep:264:108423. PMID: 40449755
| Physical Appearance | A solid |
| Storage | Store at -20°C, protect from light |
| M.Wt | 918.97 |
| Cas No. | 23109-05-9 |
| Formula | C39H54N10O14S |
| Synonyms | α-Amatoxin |
| Solubility | ≥1 mg/mL in H2O; Soluble in Ethanol |
| Canonical SMILES | CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
| Cell lines | Mouse blastocysts |
| Reaction Conditions | 1.1 μg/ml α-amanitin |
| Applications | α-Amanitin inhibited the incorporation of 3H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%. |
| Animal experiment:[1] | |
| Animal models | Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice |
| Dosage form | 0 ~ 1 μg/ml α-amanitin 48 h |
| Applications | Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos. |
| Note | The technical data provided above is for reference only. |
| References: 1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680. | |
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