Naproxen [22204-53-1]
Cat# HY-15030-1ml
Size : 10mM/1mL
Brand : MedChemExpress
Naproxen is a COX-1 and COX-2 inhibitor with IC50s of 8.72 and 5.15 μM, respectively in cell assay.
For research use only. We do not sell to patients.
Naproxen Chemical Structure
CAS No. : 22204-53-1
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This product is a controlled substance and not for sale in your territory.
- •Chemosphere. 2019 Jun;225:378-387. [Abstract]
- •Biotechnol Bioeng. 2021 Sep 3. [Abstract]
Biological Activity
Protocol
Purity & Documentation
References
Customer Review
Naproxen is a COX-1 and COX-2 inhibitor with IC50s of 8.72 and 5.15 μM, respectively in cell assay. | ||||||||||||||||
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In Vitro | Naproxen etemesil is a lipophilic, non-acidic, inactive prodrug of naproxen that is hydrolysed to pharmacologically active Naproxen once absorbed. Naproxen is a well known nonsteroidal anti-inflammatory drug. Naproxen is approximately equipotent inhibitor of COX-1 and COX-2 in intact cells with IC50s of 2.2 μg/mL and 1.3 μg/mL, respectively. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. | |||||||||||||||
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In Vivo | Naproxen exerts an anti-inflammatory and antifibrotic effect in mouse model of bleomycin-induced lung fibrosis. Naproxen also downregulates TGF-β levels and Smad3/4 complex formation. Naproxen is shown to inhibit the time-courses of pain, fever and PGE2 with similar potencies (IC50=27, 40, 13 μM). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. | |||||||||||||||
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230.26 | ||||||||||||||||
C14H14O3 | ||||||||||||||||
22204-53-1 | ||||||||||||||||
Solid | ||||||||||||||||
White to off-white | ||||||||||||||||
C[C@H](C(O)=O)C1=CC2=CC=C(OC)C=C2C=C1 | ||||||||||||||||
Room temperature in continental US; may vary elsewhere. | ||||||||||||||||
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In Vitro: DMSO : ≥ 100 mg/mL (434.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 75 mg/mL (325.72 mM; Need ultrasonic and warming) *"≥" means soluble, but saturation unknown. Preparing Stock Solutions
View the Complete Stock Solution Preparation Table * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo: Select the appropriate dissolution method based on your experimental animal and administration route. For the following dissolution methods, please ensure to first prepare a clear stock solution using an and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). | ||||||||||||||||
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BAEC are incubated for 30 min with Naproxen (0.1 ng/mL to 1 mg/mL). Arachidonic acid (30 μM) is then added, and the cells are incubated for a further 15 min at 37°C. The medium is then removed, and radioimmunoassay is used to measure the formation of 6-keto-PGF,a, PGE2, thromboxane B2, or PGF2a. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
Rats To measure the analgesic effects of naproxen in a carrageenaninduced model of monoarthritis, Male Sprague–Dawley rats (n=48, 217±28 g) are randomly divided into four groups of 12 by an internally developed computer program, allowing the blind performance of the behavioral experiment. To induce hyperalgesia by inflammation, animals in groups 1B, 1C, and 1D receive a 40-μL intra-articular injection of a saline solution containing 7.5 mg/mL carrageenan in the left hind limb under isoflurane anesthesia (time=−1 h). Animals in group 1A receive no injection. After 1 h (time=0) the animals in groups 1A, 1B, 1C, and 1D receive oral doses of naproxen in saline of 0, 0, 7.5 and 30 μmol/kg, respectively. The doses and time points of measurements are selected on the basis of simulations predicting measuring a full concentration-effect relationship within the time-span of the experiment. Mice Bleomycin (0.05 IU) is instilled intratracheally to C57BL/6 mice, which are then treated by micro-osmotic pump with vehicle, JNJ7777120 (40 mg/kg b.wt.), naproxen (21 mg/kg b.wt.), or a combination of both. Airway resistance to inflation, an index of lung stiffness, is assessed, and lung specimens are processed for inflammation, oxidative stress, and fibrosis markers. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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[1]. Mitchell JA, et al. Selectivity of nonsteroidal antiinflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11693-7.
[2]. Rosa AC, et al. Prevention of bleomycin-induced lung inflammation and fibrosis in mice by naproxen and JNJ7777120 treatment. J Pharmacol Exp Ther. 2014 Nov;351(2):308-16.
[3]. Krekels EH, et al. Pharmacokinetic-pharmacodynamic modeling of the inhibitory effects of naproxen on the time-courses of inflammatory pain, fever, and the ex vivo synthesis of TXB2 and PGE2 in rats.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O / DMSO | 1 mM | 4.3429 mL | 21.7146 mL | 43.4292 mL | 108.5729 mL |
5 mM | 0.8686 mL | 4.3429 mL | 8.6858 mL | 21.7146 mL | |
10 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL | 10.8573 mL | |
15 mM | 0.2895 mL | 1.4476 mL | 2.8953 mL | 7.2382 mL | |
20 mM | 0.2171 mL | 1.0857 mL | 2.1715 mL | 5.4286 mL | |
25 mM | 0.1737 mL | 0.8686 mL | 1.7372 mL | 4.3429 mL | |
30 mM | 0.1448 mL | 0.7238 mL | 1.4476 mL | 3.6191 mL | |
40 mM | 0.1086 mL | 0.5429 mL | 1.0857 mL | 2.7143 mL | |
50 mM | 0.0869 mL | 0.4343 mL | 0.8686 mL | 2.1715 mL | |
60 mM | 0.0724 mL | 0.3619 mL | 0.7238 mL | 1.8095 mL | |
80 mM | 0.0543 mL | 0.2714 mL | 0.5429 mL | 1.3572 mL | |
100 mM | 0.0434 mL | 0.2171 mL | 0.4343 mL | 1.0857 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
- Immunology/Inflammation Autophagy
- COX Autophagy
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Naproxen22204-53-1(S)-NaproxenCOXAutophagyCyclooxygenaseInhibitorinhibitorinhibit
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