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Reagents and instruments for immunology, cell biology and molecular biology.

Isotretinoin [4759-48-2]

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Cat# A4330-1ml

Size : 10mM(in1mLDMSO)

Contact local distributor :

Phone : +1 850 650 7790

Brand : ApexBio Technology

Contact local distributor :

Phone : +1 850 650 7790

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Background

It was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.

Chemical Properties

Storage	Store at -20°C
M.Wt	300.44
Cas No.	4759-48-2
Formula	C₂₀H₂₈O₂
Solubility	insoluble in H2O; ≥13.8 mg/mL in DMSO; ≥24.3 mg/mL in EtOH with gentle warming and ultrasonic
Chemical Name	(2Z,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoic acid
SDF	Download SDF
Canonical SMILES	CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]
Cell lines	Primary human sebocytes
Reaction Conditions	7 ~ 14 d incubation
Applications	Isotretinoin decreased sebocyte proliferation in a dose- and time-dependent manner, with IC50 values being 10 μM after a 7-d incubation and 1 μM after a 14-d incubation, respectively. Isotretinoin (8 d) also inhibited the synthesis of triglycerides, wax/stearyl esters and free fatty acids, and modulated keratin expression in primary human sebocytes at a concentration of 0.1 μM.
Animal experiment:[2]
Animal models	Male inbred Lewis (LEW, RT1¹) and Fisher (F344, RT1^1v1) rats, 200 ~ 220 g
Dosage form	2 mg/kg/day Administered orally for 8 weeks
Applications	Isotretinoin reduced chronic rejection damage and decreased mRNA expression of IFN-γ and IL-10 in allografts in chronic Fisher344➛Lewis transplant mice, an allograft nephropathy model. Thus, isotretinoin could serve as a potent immunosuppressive and anti-fibrotic agent able to prevent and inhibit progression of chronic allograft nephropathy.
Note	The technical data provided above is for reference only.
	References: 1. Zouboulis CC, Korge B, Akamatsu H, et al. Effects of 13-cis-retinoic acid, all-trans-retinoic acid, and acitretin on the proliferation, lipid synthesis and keratin expression of cultured human sebocytes in vitro. Journal of Investigative Dermatology, 1991, 96(5): 792-797. 2. Adams J, Kiss E, Arroyo AB, et al. 13-cis retinoic acid inhibits development and progression of chronic allograft nephropathy. The American Journal of Pathology, 2005, 167(1): 285-298.

Quality Control

Purity = 99.89%

Chemical structure

Isotretinoin