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Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Size : 1mL
Colchicine (Colcin) is a tubulin inhibitor (IC50: 3 nM) and blocks polymerization of microtubules by binding to tubulin.
Description | Colchicine (Colcin) is a tubulin inhibitor (IC50: 3 nM) and blocks polymerization of microtubules by binding to tubulin. |
Targets&IC50 | microtubule:3 nM |
In vitro | Colchicine (1 μM) triggered apoptosis in rat cerebellar granule cells (CGC). Colchicine treatment also causes alterations in Ca2+ responses to chemical depolarization and a moderate, but progressive, increase in the resting intracellular Ca2+ concentration[1]. Colchicine treatment inhibits indomethacin-induced small intestinal injury by 86% (1?mg/kg) and 94% (3?mg/kg) as indicated by the lesion index 24?h after indomethacin administration. Colchicine inhibits the protein expression of cleaved caspase-1 and mature IL-1β, without affecting the mRNA expression of NLRP3 and IL-1β [2]. |
In vivo | Vehicle or Colchicine (1 mg/kg) is administered orally 30 min prior to indomethacin administration. The lesions stained with Evans blue in the small intestine are smaller in Colchicine-treated mice than those in vehicle-treated mice 24 h after indomethacin administration. Colchicine treatment significantly reduces the lesion index at doses of 1 mg/kg and 3 mg/kg (by 86% and 94%, respectively) compared with vehicle treatment. Colchicine treatment significantly inhibits the protein levels of mature IL-1β at doses of 1 mg/kg and 3 mg/kg (by 56% and 69%, respectively) without affecting those of pro-IL-1β. Colchicine treatment also significantly inhibits the protein levels of cleaved caspase-1 at doses of 1 mg/kg and 3 mg/kg (by 26% and 39%, respectively) without affecting those of procaspase-1 [2]. |
Animal Research | a C57BL/6 background are used. To examine the effects of Colchicine on NSAID-induced small intestinal injury, vehicle or Colchicine (1 or 3 mg/kg) is administered orally 30 min prior to indomethacin administration. Mice received intraperitoneal injections of sterilized phosphate buffered saline or mouse recombinant IL-1β (0.1 μg/kg) 3 h after indomethacin treatment. Vehicle or Colchicine (1 or 3 mg/kg) is also administered to NLRP3?/? mice before indomethacin administration. The lesion index is evaluated 24 h after indomethacin administration and examined mRNA and protein expression of inflammasome components 6 h after indomethacin administration. |
Source |
Synonyms | Colcin, Colchineos, Colchisol |
Molecular Weight | 399.44 |
Formula | C22H25NO6 |
CAS No. | 64-86-8 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 39.9 mg/mL(100 mM)
H2O: 1.33 mg/mL(3.34 mM), sonification is recommended.