LymphoSign : CE/IVD kit for the classification of non-Hodgkin's lymphomas

LymphoSign Kit

Helps to establish an accurate diagnosis and treatment plan for non-Hodgkin's lymphoma

Non-Hodgkin's lymphoma is a type of cancer that originates in the lymphatic system, which is part of the immune system. It is characterized by the abnormal growth of lymphocytes, a type of white blood cell, and can occur in any part of the body.

There are more than 80 types of non-Hodgkin's lymphoma, some of which are rare and difficult to characterize, Hence, Accurate knowledge has become a key issue.

Many advances have been made since the introduction of the WHO classification in 2001. But the risk of errors remains higher than in other pathologies.

The LymphoSign test offers a detailed classification of these lymphomas. It is a state-of-the-art method for evaluating the stage of differentiation in non-Hodgkin's lymphoma (NHL) tumor cells. By leveraging RNA expression levels of over 130 relevant gene markers, this solution provides valuable insights into the disease progression.

This test is designed to classify the following sub-types :

High grade B-cell Non-Hodgkin's Lymphoma
- DLBCL ABC
- DLBCL GCB
- DLBCL PMBL
Low grade B-cell Non-Hodgkin's Lymphoma
- Follicular Lymphoma (FL)
- Mantle Cell Lymphoma (MCL)
- Marginal zone Lymphoma (MZL)
- Small Lymphocytic Lymphoma (SLL)

T-Cell Non-Hodgkin's Lymphoma
- Angioimmunoblastic T-cell Lymphoma Th(AITL)
- ALK-positive anaplastic large cell lymphoma (ALCL ALK+)
- ALK-negative anaplastic large cell Cytotoxic lymphoma (ALCL ALK- cytotox)
- ALK-negative anaplastic large cell Cytotoxic Th2 lymphoma (ALCL ALK- Th2)
- Adult T-cell Leukemia-Lymphoma (ATLL)
- NK/T-cell Lymphoma (NKTCL)

Use of the test

LymphoSign is an innovative, accurate, and reliable test for pathologists and molecular biologists. This test is used to characterize non-Hodgkin lymphomas (NHL).
It assesses the degree of differentiation of tumor cells by analyzing the expression level of more than 130 relevant genetic markers, based on ligation-dependent PCR technology (RT-MLPSeq). The simplicity of the test allows results to be obtained within 24 hours from tumor RNA. In addition, the protocol is optimized for low-quality samples such as FFPE samples. Using artificial intelligence trained on a database of more than 3,000 cases, the RT-MIS platform establishes the most likely classification among 13 subtypes of B-cell and T-cell NHL.

A simple and fast protocol

No purification required until library preparation, minimizing material loss and maintaining high sensitivity.
Short genetic sequences targeted (40-60 bases), ensuring robustness against RNA degradation.
Particularly suitable for analyzing difficult biological samples like paraffin-fixed tissue biopsies.
10⁵ sequences per sample sufficient for analyzable expression profiles, allowing high-throughput analysis.
Libraries can be loaded simultaneously with other sequencing libraries, optimizing cost efficiency.

Precise computer analysis based on dedicated software

Once sequencing has been completed, the FASTQ file can be uploaded to the RT-MIS platform. which, after just a few minutes of analysis a report with the expression signature of the various genes and an AI-predicted classification according to the 2017 WHO classification, offering users a complete solution.

Handling duration	≃4h
Actual working time	≃1h-1h30
Type of Nucleic acid	RNA
Input quantity	Between 50 and 500ng of RNA in a volume of 2μl
Type of cancer	Non-hodgkin lymphoma
Contents of the reagent kit	Probes targeting 137 markers, barcodes, sequence primers
Method	Ligation dependent RT-PCR
Description	Compare the expression profiles obtained from genes, somatic mutations or chromosomal translocations with those of the main types of non-Hodgkin's lymphoma.
Equipment compatibility	MiSeq, NextSeq 500, NextSeq 550 Illumina®
Type of samples	Tissue biopsies at room temperature, frozen or fixed and included in paraffin
Technology	Next Generation Sequencing

Thanks to the LymphoSign Test-Kit, the classification of lymphoma has become a simple process.

Now, you can Purchase the Kit that matches your needs and be sure to obtain the best results in a short time

Description	Cat#	Cond.
LymphoSign Test-Kit	GEP-LS08	8 reactions
	GEP-LS16	16 reactions
	GEP-LS24	24 reactions
	GEP-LS48	48 reactions

For more information:

Consult the reference publication LymphoSign Test-Kit

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These products are medical devices.

Please follow the manufacturer's instructions