Trident RIPA Lysis Buffer
Cat. No. GTX400005
Cat. No. GTX400005
GTX400005 WB Image
A431 and HeLa cells were lysed by using GTX400005 RIPA lysis buffer.
Whole cell extracts (30 μg) were assayed by Western Blot analysis.
The membrane was blotted with a cytosolic protein GAPDH antibody (GTX627408), a membrane protein Flotillin1 antibody (GTX104769) and a nuclear protein LaminB2 (GTX628803).
GTX400005 Image
Summary
Radio-Immunoprecipitation assay lysis buffer
The ingredients of RIPA lysis buffer are as follows:
25 mM Tris pH 7.6
150 mM NaCl
1% Triton X-100
1% sodium deoxycholate
1% SDS
The ingredients of RIPA lysis buffer are as follows:
25 mM Tris pH 7.6
150 mM NaCl
1% Triton X-100
1% sodium deoxycholate
1% SDS