Tricine Buffer 0.5M, pH 7.5
Cat# 42020341-2
Size : 250mL
Brand : bioWORLD
Description
Tricine or N-[Tris(hydroxymethyl)methyl]glycine is a zwitterionic amino acid and is one of the original Good's buffers. It is a buffering agent in the separation of low molecular weight peptides. Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement. Tricine provides high clarity resolution and is very soluble in water.
Properties
Shelf life
5 years
Storage Temperature
RT
ECCN #
EAR99
Concentration
0.5M
Appearance color
Clear
Appearance form
Liquid
Molecular Formula
C6H13NO5
Molecular Weight
179.17
pH value
7.5
pH Range
7.4-8.8
Density
1.45 g/cm3
Melting point
187°C
Boiling point
500.92C
Solubility (@ RT)
Soluble in water
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