TPO, Recombinant, Rat, Animal Free (Rat Thrombopoietin, Megakaryocyte Colony-Stimulating Factor, c-MPL Ligand, MGDF)

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle.It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo.TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets.Human and murine TPO exhibits cross-species reactivity.Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kD), which contains the erythropoietin-like domain of the full length TPO protein.

Manufactured using all Animal-Free reagents.

Recombinant protein corresponding to rat TPO expressed in E. coli. Swiss/Uniprot: P49745

Biological Activity:
Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is <0.2ng/ml, corresponding to a specific activity of >5x10e6units/mg.

Amino Acid Sequence:
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.