Western Blot (Cell lysate)
Western Blot analysis of TNFRSF1A expression from cell extracts with TNFRSF1A polyclonal antibody (Cat # PAB12277).
Lane 1 : HT1080 whole cell lystae.
Lane 2 : smmc cell lysate.
Lane 3 : MM453 whole cell lysate.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TNFRSF1A.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human TNFRSF1A.
Host
Rabbit
Theoretical MW (kDa)
50
Reactivity
Goat, Human, Mouse, Rat
Specificity
Different to the related rat sequence by a single amino acid.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot (Cell lysate)
Western Blot analysis of TNFRSF1A expression from cell extracts with TNFRSF1A polyclonal antibody (Cat # PAB12277).
Lane 1 : HT1080 whole cell lystae.
Lane 2 : smmc cell lysate.
Lane 3 : MM453 whole cell lysate. - Gene Info — TNFRSF1A
Entrez GeneID
7132Gene Name
TNFRSF1A
Gene Alias
CD120a, FPF, MGC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60
Gene Description
tumor necrosis factor receptor superfamily, member 1A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq
Other Designations
tumor necrosis factor binding protein 1|tumor necrosis factor receptor 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Tumor necrosis factor alpha-receptor type 1 activation in the hypothalamic paraventricular nucleus contributes to glutamate signaling and angiotensin II-dependent hypertension.
Clara Woods, Jose Marques-Lopes, Natalina H Contoreggi, Teresa A Milner, Virginia M Pickel, Gang Wang, Michael J Glass.
Journal of Neuroscience 2021 Feb; 41(6):1349.
Application：IEM, IHC, Mouse, Mouse brain.
- Ultrastructural characterization of tumor necrosis factor alpha receptor type 1 distribution in the hypothalamic paraventricular nucleus of the mouse.
Glass MJ, Chan J, Pickel VM.
Amino Acids 2017 Apr; 352:262.
Application：ICC, Mouse, Mouse brain.
- Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes.
Micheau O, Tschopp J.
Cell 2003 Jul; 114(2):181.
Application：Func, Human, HT-1080 cells.
- MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase.
Schievella AR, Chen JH, Graham JR, Lin LL.
The Journal of Biological Chemistry 1997 May; 272(18):12069.
Application：IP, Monkey, COS cells.
- Structure of the human TNF receptor 1 (p60) gene (TNFR1) and localization to chromosome 12p13 [corrected].
Fuchs P, Strehl S, Dworzak M, Himmler A, Ambros PF.
Genomics 1992 May; 13(1):219.
- Tumor necrosis factor alpha-receptor type 1 activation in the hypothalamic paraventricular nucleus contributes to glutamate signaling and angiotensin II-dependent hypertension.