Reagents and instruments for immunology, cell biology and molecular biology.
 
> TIMP3 ELISA Kit (Horse)

TIMP3 ELISA Kit (Horse)

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Cat# OKBB00747

Size : 96wells/kit,withremovablestrips.

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

TIMP3 ELISA Kit (Horse) (OKBB00747)

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ELISA standard curve
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHorse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's Equine TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NS0, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Equine TIMP-3 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)125026875353143028325657
Standard deviation72.5110.2192.795.81147.3277.2
CV(%)5.84.13.66.75.24.9
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Horse TIMP3 Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Horse TIMP3 Standard2 x 10 ng
100X Biotinylated Anti-Horse TIMP3 Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123326026124324911.794.7%
Sample 21357134213731251133047.333.5%
Sample 343833655422043204144288.526.9%

*number of samples for each test n=16.

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Standard Number87654321
Concentration (pg/ml)015631262512500025000500010000
O.D.0.0080.0520.0790.1600.3560.8261.6932.485
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeSandwich High Sensitivity ELISA kit for Quantitative Detection of horse equine TIMP-3
Sensitivity<2 pg/ml
ImmunogenExpression system for standard: NS0; Immunogen sequence: C24-P211
Gene SymbolTIMP3
Gene Full NameMetalloproteinase inhibitor 3
Alias SymbolsMetalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3;
NCBI Gene Id100033947
Protein NameMetalloproteinase inhibitor 3
Description of TargetThe tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).
Uniprot IDP35625
Protein Accession #NP_000353.1
Nucleotide Accession #NM_000362.4