Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit
Cat# E-BC-K298-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit (E-BC-K298-M)
For research use only.
| Detection Principle | TBARS can react with TBA under high temperature and acid conditions to form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the OD values at 530-540 nm. |
| Synonyms | TBARS |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-540 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.85 μmol/L |
| Detection Range | 2.6-100 μmol/L |
| Precision | inter-assay CV: 7.1% | intra-assay CV: 4.3% |
| Sample Volume | 0.1 mL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| Human serum | 1 |
| Rat serum | 1 |
| 10% Rat liver tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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