TET3 Antibody - C-terminal
Cat# OAPB01637
Size : 0.1mg
Brand : Aviva Systems Biology
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TET3 Antibody - C-terminal (OAPB01637)
| Datasheets/Manuals | Printable datasheet for TET3 Antibody - C-terminal (OAPB01637) (OAPB01637) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
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| Product Format | Liquid. TET3 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | ELISA, IF, LFA, WB |
| Reconstitution and Storage | Store at 4C for 3 months or -20C for up to 1 year. Avoid repeated freeze thaw cycles. Do not expose to prolonged high temperatures. |
| Immunogen | TET3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TET3. The immunogen is located within amino acids 1520 - 1570 of TET3. |
| Purification | Affinity Purified |
| Concentration | 1 mg/ml |
| Specificity | At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins. |
| Application Info | WB 1 ug/ml IHC 2.5 ug/ml IF 20 ug/ml |
| Reference | 1) Langemeijer SM, Kuiper RP, Berends M, et al. Acquired mutations in TET2 are common in myelodysplastic syndromes. Nat. Genet. 2009; 41:838-42. 2) Schaub FX, Looser R, Li S, et al. Clonal analysis of TET2 and JAK2 mutations suggests that TET2 can be a late event in the progression of myeloproliferative neoplasms. Blood 2011; 115:2003-7. 3) Langemeijer SMC, Aslanyan MG, and Jansen JH. TET proteins in malignant hematopoiesis. Cell Cycle 2009; 4044-8. 4) Gu TP, Guo F, Yang H, et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 2011; 477:606-10. |
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| Gene Symbol | TET3 |
| Gene Full Name | tet methylcytosine dioxygenase 3 |
| Alias Symbols | BEFAHRS;hCG_40738;methylcytosine dioxygenase TET3;probable methylcytosine dioxygenase TET3;putative methylcytosine dioxygenase;ten-eleven translocation 3;tet oncogene family member 3. |
| NCBI Gene Id | 200424 |
| Protein Name | Methylcytosine dioxygenase TET3|methylcytosine dioxygenase TET3 isoform 1 [Homo sapiens] |
| Description of Target | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707). In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889). |
| Uniprot ID | O43151 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_001274420 |
| Molecular Weight | 183 kDa |