TET1 Antibody - C-terminal
Cat# OAPB01879
Size : 0.1mg
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
TET1 Antibody - C-terminal (OAPB01879)
| Datasheets/Manuals | Printable datasheet for TET1 Antibody - C-terminal (OAPB01879) (OAPB01879) |
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| Predicted Species Reactivity | Bovine|Human|Mouse|Porcine|Rat |
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| Product Format | Liquid. TET1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | ELISA, IF, LFA |
| Reconstitution and Storage | Store at 4C for 3 months or -20C for up to 1 year. |
| Immunogen | Anti-TET1 antibody (7733) was raised against an 18 amino acid peptide near the carboxy terminus of human TET1. The immunogen is located within amino acids 2030 - 2080 of TET1. |
| Purification | Affinity Purified |
| Concentration | 1 mg/ml |
| Specificity | TET1 antibody is predicted to not cross-react with TET2 and TET3. |
| Application Info | WB 1-2 ug/ml IHC 5 ug/ml IF 20 ug/ml |
| Reference | 1) Lorsbach RB, Moore J, Mathew S, et al. TET1, a member of a novel protein family, is fused to MLL in acute myeloid leukemia containing the t(10;11)(q22;q23). Leukemia 2003; 17:637-41. 2) Tahilani M, Koh KP, Shen Y, et al. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 2009; 324:930-5. 3) Neves-Costa A and Moita LF. TET1 is a negative transcriptional regulator of IL-1b in the THP-1 cell line. Mol. Immunol. 2013; 54:264-70. 4) Sun M, Song CX, Huang H, et al. HMGA2/TET1/HOXA9 signaling pathway regulates breast cancer growth and metastasis. Proc. Natl. Acad. Sci. USA 2013; 110:9920-5. |
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| Gene Symbol | TET1 |
| Gene Full Name | tet methylcytosine dioxygenase 1 |
| Alias Symbols | bA119F7.1;CXXC finger 6;CXXC zinc finger 6;CXXC6;CXXC-type zinc finger protein 6;LCX;leukemia-associated protein with a CXXC domain;methylcytosine dioxygenase TET1;ten-eleven translocation 1 gene protein;ten-eleven translocation-1;tet oncogene 1;TET1 splice variant VP_DE4;TET1 splice variant VP_DE456. |
| NCBI Gene Id | 80312 |
| Protein Name | Methylcytosine dioxygenase TET1|methylcytosine dioxygenase TET1 [Homo sapiens] |
| Description of Target | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an important role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). |
| Uniprot ID | Q8NFU7 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_085128 |
| Nucleotide Accession # | NM_030625.2 |
| Molecular Weight | Predicted: 235 kDa Observed: 235 kDa |
