A8005-100mg | Sulfo-NHS-SS-Biotin [325143-98-4] Quimigen Portugal

Sulfo-NHS-SS-Biotin

Catalog No.

A8005

Sulfo-NHS-SS-Biotin

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Background
Product Citation
Features
Chemical Properties
Protocol
Biological Activity
Quality Control
User Guide

Background

Sulfo-NHS-SS-Biotin is an amine-reactive biotinylation reagent designed for labeling molecules containing primary amines. This compound possesses a negatively charged sulfonate group, imparting enhanced aqueous solubility and enabling its direct use in aqueous reaction systems without organic solvents. Its sulfo-NHS ester is unstable in solution, necessitating fresh preparation and immediate use to avoid hydrolysis. Post-conjugation, it forms stable complexes with amine targets, enabling purification/detection via avidin/streptavidin affinity chromatography. It is widely used in biochemical research for protein labeling, affinity purification, and bioconjugation.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

Product Citation

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Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Chemical Properties

Storage	Store at -20°C The product is not stable in solution, please dissolve it immediately before use.
M.Wt	606.7
Cas No.	325143-98-4
Formula	C₁₉H₂₇N₄NaO₉S₄
Synonyms	Biotin disulfide N-hydroxysulfosuccinimide ester
Solubility	≥30.33 mg/mL in DMSO; <2.68 mg/mL in EtOH; <2.74 mg/mL in H2O
Chemical Name	sodium;1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
Canonical SMILES	C1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Biotinylation method [1]:
Sample	468/EV cells and 468/uPAR cells.
Preparation method	Soluble in water, DMSO or DMF.
Reaction Conditions	1 mg/ml, 15minutes on ice
Applications	Cells in monolayer culture (1.5 × 106) were washed three times with ice-cold PBS and then treated with sulfo-NHS-SS-biotin (1mg/mL) for 15 minutes on ice. Biotinylation reactions were terminated with 100 mmol/L glycine in PBS. After washing with PBS, cell extracts were prepared in radioimmunoprecipitation assay (RIPA) buffer (20 mmol/L sodium phosphate, 150 mmol/L NaCl (pH 7.4), 1% NP40, 0.1% SDS, and 0.5% deoxycholic acid) with protease inhibitor cocktail. Biotinylated membrane proteins were precipitated with streptavidin-sepharose. Proteins were eluted with SDS sample buffer, resolved by SDS-PAGE, electrotransferred to polyvinylidene difluoride (PVDF) membranes, and probed with primary antibodies.
References: [1]. Minji Jo, Boryana M. Eastman, Drue L. Webb, et al. Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell-like Properties in Breast Cancer cells . Cancer Res, 2010;70:8948-8958