600333 | Soluble TNFR1 Quimigen Portugal

Recombinant Active Protein

Cat. No.

600333

Unit

0.02 mg

Description

TNF Receptor 1 (TNFR1) is expressed in most tissues and is activated by soluble and membrane-bound TNFalpha. TNFR1 is known to activate NF-kB and MAPK pathways to induce inflammation, promote apoptotic cell death, inhibit tumorigenesis and inhibit viral replication. Recombinant soluble TNFR1 is produced in E.coli. The soluble form of recombinant human TNFR1 is a non-glycosylated protein, containing 162 amino acids, with a molecular weight of 18.3 kDa.

Spec Container

Datasheet

Format

Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Alternate Names

Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p60; p55; CD120a; TNFRSF1A; TNFAR; TNFR1

Accession No.

P19438

18300 Da

Sequence

MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESSGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

Source

E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

The activity is determined by its ability to inhibit the cytolytic effects 0.25 ng/mL TNF? has on mouse L929 cells, in the presence of Actinomycin D, and is typically between 0.045-0.09 ng/mL. Specific activity: 2 x 10e7 units/mg

Storage

Store at -20C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.

Images

For research use only, not for diagnostic or therapeutic procedures.

Soluble TNFR1

Cat# 600333

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Brand : Abbiotec

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Spec Container