SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag

Cat# GTX136905-pro

Size : 100ug

Brand : Genetex

Contact local distributor :


Phone : +1 850 650 7790

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

Application

WB, ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg
View product citations for antibody GTX136905-pro on CiteAb

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX635807, Detection : GTX635792-01.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1 mg/ml. Lyophilized from PBS.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H mutations, and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Omicron variant Spike , BA.2 Spike , SARS-CoV-2 Omicron variant Spike , COVID-19 Omicron variant Spike

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (GTX136905-pro) (143.73-2.25 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (GTX136905-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro Image

GTX136905-pro SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD Protein, BA.2 / Omicron variant, His tag (1 μg, GTX136905-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD Protein, Omicron / BA.2 variant, His tag. GTX136905-pro

GTX136905-pro ELISA Image

Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

Application Reference

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens
Application : ELISA

REVIEW

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Application Reference
TW Liu et al. J Ethnopharmacol 2024; 320:117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
CL Ho et al. Front Immunol 2023; Long-term analysis of humoral responses and spike-specific T cell memory to Omicron variants after different COVID-19 vaccine regimens
Application : ELISA