RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
Cat# MBS652918-10L
Size : 10L
Brand : MyBiosource
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose Culture Media
For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS652918RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et al. at Roswell Park Memorial Institute. More details
Product Overview
Online Datasheet in PDFProduct Name
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Culture Media
Full Product Name
RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.
Appearance
Yellow, homogeneous, free flowing powder
pH
8.5
Solubility
Magenta, clear, complete
Endotoxin
<1EU/ml
Directions per Liter
Dissolve 7.40g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/liter
Inorganic Salts:
Calcium Nitrate•4H2O: 0.1
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.4
Sodium Chloride: 6
Sodium Phophate Dibasic: 0.8
Amino Acids:
L-Arginine: Absent
L-Aspartic Acid: Absent
L-Asparagine: Absent
L-Cystine•2HCl: Absent
L-Glutamic Acid: Absent
L-Glutamine: Absent
Glycine: Absent
L-Histidine: Absent
Hydroxy-L-proline: Absent
L-Isoleucine: Absent
L-Leucine: Absent
L-Lysine•HCl: Absent
L-Methionine: Absent
L-Phenylalanine: Absent
L-Proline: Absent
L-Serine: Absent
L-Threonine: Absent
L-Tryptophan: Absent
L-Tyrosine•2Na•2H2O: Absent
L-Valine: Absent
Vitamins:
P-Amino Benzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Vitamin B-12: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid•Ca: 0.00025
Pyridoxine•HCl: 0.001
Riboflavin: 0.0002
Thiamine•HCl: 0.001
Other:
Glucose: Absent
Glutathione: 0.001
Phenol Red, Sodium: 0.0053
Total: 7.40g/liter
Calcium Nitrate•4H2O: 0.1
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.4
Sodium Chloride: 6
Sodium Phophate Dibasic: 0.8
Amino Acids:
L-Arginine: Absent
L-Aspartic Acid: Absent
L-Asparagine: Absent
L-Cystine•2HCl: Absent
L-Glutamic Acid: Absent
L-Glutamine: Absent
Glycine: Absent
L-Histidine: Absent
Hydroxy-L-proline: Absent
L-Isoleucine: Absent
L-Leucine: Absent
L-Lysine•HCl: Absent
L-Methionine: Absent
L-Phenylalanine: Absent
L-Proline: Absent
L-Serine: Absent
L-Threonine: Absent
L-Tryptophan: Absent
L-Tyrosine•2Na•2H2O: Absent
L-Valine: Absent
Vitamins:
P-Amino Benzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Vitamin B-12: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid•Ca: 0.00025
Pyridoxine•HCl: 0.001
Riboflavin: 0.0002
Thiamine•HCl: 0.001
Other:
Glucose: Absent
Glutathione: 0.001
Phenol Red, Sodium: 0.0053
Total: 7.40g/liter
Important Note
Hygroscopic. Keep tightly closed and desiccated
Product Categories/Family for RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose culture media
Culture Media; Media-Cell Culture; RPMI
Other Notes
Small volumes of RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose purchase
MBS652918 is a culture media targeting RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose. Also known as RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder).
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