Recombinant Proteinase K

Cat# HY-P3150-50mg

Size : 50mg

Brand : MedChemExpress

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Description

Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].

In Vitro

Proteinase K digestion[1]
1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.
2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 μl aliquot to get the final concentrations of 2, 4 and 8 μg/ml. Reactions are carried out at 37°C for 30 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Color

Off-white to light yellow

SMILES

[Recombinant Proteinase K]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References