Rat IgG (H+L), Mouse ads Antibody : AP
Cat# OASB02189
Size : 1.0mL
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Donkey Anti-Rat IgG(H+L) Antibody - AP Conjugated (OASB02189)
| Datasheets/Manuals | Printable datasheet for Donkey Anti-Rat IgG(H+L) Antibody - AP Conjugated (OASB02189) |
|---|
| Tested Species Reactivity | Rat |
|---|---|
| Predicted Species Reactivity | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Donkey |
| Conjugation | AP |
| Application | IHC-P, ICC, WB |
| Additional Information | Description: Pooled antisera from donkeys hyperimmunized with rat IgG; purified by affinity chromatography on rat IgG covalently linked to agarose; reacts with the heavy and light chains of rat IgG and the light chains of rat IgM; cross-adsorbed against mouse serum proteins (SP) for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rat IgG |
| Purification | Affinity chromatography on Rat IgG covalently linked to agarose. |
| Concentration | Lot specific |
| Specificity | IgG(H+L) |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA and/or FLISA for conformance tocharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | FLISA: ELISA: FITC conjugate R-PE conjugate AP conjugate HRP conjugate BIOT conjugate 1:200 – 1:400 1ug 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Western blotting, Dot-and slot-immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Minimal cross reaction to mouse serum proteins |
| Gene Full Name | Ig gamma-1 chain C region |
|---|
