- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAD9.
Immunogen
A synthetic peptide corresponding to amino acids 1249-1263 residues surrounding S1260 of Saccharomyces cerevisiae RAD9.
Host
Rabbit
Reactivity
Yeast
Specificity
No reactivity is expected against the human analog of RAD9.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:60000 of phosphorylated form 1:6000 of non-phosphorylated form)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot
- Gene Info — RAD9
Entrez GeneID
851803Gene Name
RAD9
Gene Alias
-
Gene Description
DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate
Gene Ontology
HyperlinkGene Summary
-
Other Designations
Rad9p
- Publication Reference
- Reconstitution and molecular analysis of the hRad9-hHus1-hRad1 (9-1-1) DNA damage responsive checkpoint complex.
Burtelow MA, Roos-Mattjus PM, Rauen M, Babendure JR, Karnitz LM.
The Journal of Biological Chemistry 2001 Jul; 276(28):25903.
Application:IP, IP-WB, WB-Tr, Human, Insect, K-562, Sf9 cells.
- Mutant alleles of Schizosaccharomyces pombe rad9(+) alter hydroxyurea resistance, radioresistance and checkpoint control.
Hang H, Rauth SJ, Hopkins KM, Lieberman HB.
Nucleic Acids Research 2000 Nov; 28(21):4340.
- HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins.
Cai RL, Yan-Neale Y, Cueto MA, Xu H, Cohen D.
The Journal of Biological Chemistry 2000 Sep; 275(36):27909.
Application:WB-Tr, Monkey, COS-7 cells.
- Reconstitution and molecular analysis of the hRad9-hHus1-hRad1 (9-1-1) DNA damage responsive checkpoint complex.
RAD9 polyclonal antibody
Cat# PAB11363
Size : 100ug

