Rabbit Anti-SUCLG1 Polyclonal Antibody

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Cat# HPA036683-25ul

Size : 25ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

HPA036683

succinate-CoA ligase, alpha subunit

Anti-SUCLG1 Antibody

Polyclonal Antibody against HUMAN SUCLG1

0.2 mg/ml

info@quimigen.pt

Immunohistochemical staining of human cerebellum, fallopian tube, kidney and stomach using Anti-SUCLG1 antibody HPA036683 (A) shows similar protein distribution across tissues to independent antibody HPA036684 (B).

Product details

Target protein: succinate-CoA ligase, alpha subunit
Target gene: SUCLG1
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000052738 (91%)RAT - ENSRNOG00000005587 (88%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000002051
Concentration: 0.2 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:1000 - 1:2500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: succinate-CoA ligase, alpha subunit
Gene name: SUCLG1
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas