Rabbit Anti-SLC8A1 (Center) Polyclonal Antibody
Cat# AP8939C-ev
Size : 80ul
Brand : Abcepta
Contact local distributor :
Phone : +1 850 650 7790
SLC8A1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Western blot analysis of SLC8A1 Antibody (Center) (Cat. #AP8939c) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.
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SLC8A1 Antibody (Center) (Cat. #AP8939c) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC8A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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SLC8A1 Antibody (Center) (Cat. #AP8939c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SLC8A1 Antibody (Center) (Cat. #AP8939c) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.
Application 
| WB, FC, IHC-P, E |
|---|---|
| Primary Accession | P32418 |
| Other Accession | Q01728, P70414, P48765 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 108547 Da |
| Antigen Region | 296-325 aa |
| Gene ID | 6546 |
|---|---|
| Other Names | Sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Solute carrier family 8 member 1, SLC8A1, CNC, NCX1 |
| Target/Specificity | This SLC8A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-325 amino acids from the Central region of human SLC8A1. |
| Dilution | WB~~1:1000 FC~~1:10~50 IHC-P~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SLC8A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SLC8A1 |
|---|---|
| Function | Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:11241183, PubMed:1374913, PubMed:1476165). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:11241183, PubMed:1374913, PubMed:1476165). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum (PubMed:11241183, PubMed:1374913, PubMed:1476165). SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:11241183, PubMed:1374913, PubMed:1476165). Required for normal embryonic heart development and the onset of heart contractions (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Detected primarily in heart and at lower levels in brain (PubMed:1374913). Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung (PubMed:1476165) |
- Primary Antibodies
- Cancer
- Cardiovascular
- Metabolism
- Signal Transduction