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> Rabbit Anti-RUNX2 Polyclonal Antibody

Rabbit Anti-RUNX2 Polyclonal Antibody

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Cat# HPA022040

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA022040
runt-related transcription factor 2

Anti-RUNX2 Antibody

Polyclonal Antibody against HUMAN RUNX2

0.1 mg/ml
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
Anti-RUNX2 Antibody
info@quimigen.pt

Immunohistochemistry analysis in human tonsil and liver tissues using HPA022040 antibody. Corresponding RUNX2 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: runt-related transcription factor 2
  • Target gene: RUNX2
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000039153 (100%)RAT - ENSRNOG00000020193 (81%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000051625
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. 

Target information

  • Protein name: runt-related transcription factor 2
  • Gene name: RUNX2
  • Alternative gene names: AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (18)

  • Saturne MD, Motch Perrine SM, Li Q, Richtsmeier JT, Jabs EW, van Bakel H, Holmes GDevelopment , 2025 Oct 13; 152(19):dev204875. Epub 2025 Oct 132025 Oct 13
  • Chen Y, Zhang Y, Chen X, Huang J, Zhou B, Zhang T, Yin W, Fang C, Yin Z, Pan H, Li X, Shen W, Chen XAdv Sci (Weinh) , 2023 Oct 23; 10(34):2304216. Epub 2023 Oct 232023 Oct 23